Sr. Research Laboratory Technician
University of Miami

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Sr. Research Laboratory Technician - Yeager Lab, The Frost Institute for Chemistry and Molecular Science

The Yeager lab is intrigued by biological questions at the interface between cell biology and structural biology. How do membrane channels open and close? How are signals transmitted across a cellular membrane when an extracellular ligand binds to a membrane receptor? How do viruses attach and enter host cells, replicate and assemble infectious particles? We use high resolution electron cryo-microscopy (cryo-EM) and image processing to explore the molecular design of large, multicomponent supramolecular assemblies. Biological specimens are quick frozen in a physiological state to preserve their native structure and functional properties. A special advantage of this rapid-freezing method is that we can trap and image dynamic states of functioning macromolecular assemblies, such as open and closed states of membrane channels and viruses actively transcribing RNA. Three-dimensional density maps are obtained by digital image processing of the high-resolution electron micrographs. The rich detail in the maps reveals the structural organization of complex biological structures that can be related to the functional properties of such assemblies.

The University of Miami is offering an excellent job opportunity for a Senior Research Technician position at the Frost Institute for Chemistry and Molecular Science within the laboratory of Dr. Mark Yeager. We are looking for a talented Sr. Research Laboratory Technician who will serve as key personnel on our current research projects. This individual will conduct research experiments in accordance with laboratory and safety protocols and will collaboratively and independently perform research laboratory techniques and procedures. The Sr. Research Laboratory Technician performs advanced technical activities related to laboratory research projects within the department. Specifically, the Sr. Research Laboratory Technician aids scientists by performing experiments, monitoring and recording study findings.

CORE JOB FUNCTIONS

• Maintains laboratory cell cultures, including mammalian, bacterial and insect cells.
• Under general supervision performs complex laboratory techniques including, but not limited to recombinant protein purification, DNA purification, flow cytometry.
• Cleans, sanitizes, maintains, and operates laboratory equipment and glassware.
• Maintains laboratory supply inventory and assists with ordering.
• Performs data collection, data management and analysis using laboratory/computer equipment.
• Uses biological safety cabinets, balances, pH meter, laboratory instruments such as laminar flow hoods, centrifuges, FPLC systems, flow cytometer.
• Maintains accurate records of procedures used to conduct experiments.
• Contributes to report writing and graphical presentation of data.
• Completes all required documentation for laboratory requests.
• Schedules laboratory work based on project priorities to meet deadlines. Adheres to University and unit-level policies and procedures and safeguards University assets.

This list of duties and responsibilities is not intended to be all-inclusive and may be expanded to include other duties or responsibilities as necessary.

CORE QUALIFICATIONS

Education:

High school diploma or equivalent. Bachelor's degree in biological sciences or related discipline (physics, chemistry) preferred.

Certification and Licensing:

Not Applicable

Experience:

Minimum 3 years relevant experience working in a research laboratory performing laboratory procedures in the areas of biochemistry, molecular biology, microbiology or cell biology. Any appropriate combination of relevant education, experience and/or certifications may be considered.

Knowledge, Skills and Attitudes:

• Skill in completing assignments accurately and with attention to detail.
• Ability to analyze, organize and prioritize work under pressure while meeting deadlines.
• Ability to process and handle confidential information with discretion.
• Ability to work evenings, nights, and weekends as necessary.
• Commitment to the University's core values.
• Ability to work independently and/or in a collaborative environment.
• Proficiency in computer software (i.e., Microsoft Office).

The University of Miami is an Equal Opportunity Employer - Females/Minorities/Protected Veterans/Individuals with Disabilities are encouraged to apply. Applicants and employees are protected from discrimination based on certain categories protected by Federal law. Click here for additional information.

Job Status:
Full time

Employee Type:
Staff

Pay Grade:
A6

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