Technician Research Assistant II DOM/Channing
Brigham & Women's Hospital(BWH)

Boston, Massachusetts

This job has expired.

GENERAL SUMMARY/ OVERVIEW STATEMENT: Summarize the nature and level of work performed.

The Respiratory Functional Genetics Laboratory focuses on biological functional evaluation of genetic variants associated with asthma and chronic obstructive pulmonary disease (COPD). The TRAII will be an active participant in experimental research and will have the opportunity to present his/her lab work. He/she will work independently, and perform non-routine, highly specialized molecular biology experiments. The TRAII will also participate in the modification and development of new research protocols.

PRINCIPAL DUTIES AND RESPONSIBILITIES: Indicate key areas of responsibility, major job duties, special projects and key objectives for this position. These items should be evaluated throughout the year and included in the written annual evaluation.

1) Independently perform molecular biology and cell biology procedures including but not limited to DNA and cDNA cloning, RNA isolation, cell transformation and transfection, mammalian and bacterial cell culture, reporter assay, florescence microscopy, and validation by rtPCR and western blot.

2) Responsible for the coordination of individual projects, including quality assurance and scheduling to meet deadlines.

3) Participate and eventually lead some bioinformatic analysis, including but not limited to RNA-seq analysis and gene expression correlation analysis;

3) Works independently and in teams to perform experiments and analyze results.

4) Present results from experiments, bioinformatic analysis in the lab meeting.

5) Establish new and modify existing research techniques.

6) Present journal club paper indecently after thoughtful reading literatures.

7) Assist Senior Research Technician with lab organization and reagents consolidation.

8)All other duties as assigned.

QUALIFICATIONS: (MUST be realistic, neither overstated nor understated, and related to the essential functions of the job.)

  • BS or BA required; MS degree preferred in Biological Science.
  • Minimum 1 year of relevant lab work experience.
  • Experience with molecular cloning, PCR, mini and maxi preparation plasmid, cell culture, transfection, western blot, immunoprecipitation and immunofluorescence.
  • Experience with nuclear protein extraction, reporter assay and RNAi delivery is plus.
  • Experience with mouse models would be desirable.

  • SKILLS/ ABILITIES/ COMPETENCIES REQUIRED: (MUST be realistic, neither overstated nor understated, and related to the essential functions of the job.)

    1) Ability to attend to details and to be precise. Sound analytical and organizational skills.

    2) Excellent written and verbal communication skills; must be able to thoroughly document experiments, protocols, and data. Must be able to logically structure tasks and set priorities.

    3) Knowledge of cell biology and/or molecular biology typically obtained through a bachelor degree program in biology, molecular genetics, biochemistry, or laboratory science. Broad knowledge of scientific principles and laboratory techniques. Ability to identify potential problems, communicate, and troubleshoot them effectively.

    4) Be able to operate and maintain delicate electronic equipment and safely use and dispose of toxic chemicals and biohazardous materials.

    EEO Statement
    BWH is an Affirmative Action Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment.

    This job has expired.

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